Coding

Part:BBa_K4171004:Design

Designed by: HUANG, YEN-JUNG   Group: iGEM22_NCKU_Tainan   (2022-08-11)


CysE*


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 690
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We induce five mutations into the original cysE sequence, in order to allow it not to be inhibited by excessive cysteine or selenocysteine, and thus increase the final production.

Mutations details: p.T167A, G203S, T234S, P252L, M256Q

Source

CysE* derives from Escherichia coli

References